Isolation of trypsins by affinity chromatography.

To learn the molecular mechanism of an enzyme action, first the protein has to be purified. Because of its simplicity and reproducibility, affinity chromatography has become a adopted method throughout the scientific community. Affinity chromatography occupies a unique place in separation technology since it is the only technique which enables purification of almost any biomolecules on the basis of its biological function or individual chemical structure. The principle of the affinity chromatography is shown in Figure 1. and Figure 2.